Novel real‐time PCR detection assay for Brucella suis
نویسندگان
چکیده
منابع مشابه
Novel real-time PCR detection assay for Brucella suis
INTRODUCTION Brucella suis is the causative agent of brucellosis in suidae and is differentiated into five biovars (bv). Biovars 1 and 3 possess zoonotic potential and can infect humans, whereas biovar 2 represents the main source of brucellosis in feral and domestic pigs in Europe. Both aspects, the zoonotic threat and the economic loss, emphasize the necessity to monitor feral and domestic pi...
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The routine identification and differentiation of Brucella species is a time-consuming and labor-intensive process, which frequently places personnel at risk of laboratoryacquired infection. Here, we describe the development of a rapid multiplex PCR assay for the confirmation of presumptive Brucella isolates. The assay was able to identify and differentiate major human pathogens, namely B. abor...
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Background: Classical bacteriological detection of Brucella species from food, and environment is routinely carried out based on morphological and biochemical characteristics. However, for increasing specificity and sensitivity of species identification methods, development of a molecular assay is necessary that was main aim of this study. Methods: Panel of some reference strains belonging to ...
متن کاملA novel real-time PCR assay for specific detection of Brucella melitensis
BACKGROUND Brucellosis is a zoonosis that occurs worldwide. The disease has been completely eradicated in livestock in Sweden in 1994, and all cases of confirmed human brucellosis are imported into Sweden from other countries. However, due to an increase in the number of refugees and asylum seekers from the middle-east to Sweden, there is a need to improve the current diagnostic methodology for...
متن کاملA Novel PCR Assay for Detecting Brucella abortus and Brucella melitensis
OBJECTIVES Brucellosis is a major zoonotic disease that poses a significant public health threat worldwide. The classical bacteriological detection process used to identify Brucella spp. is difficult and time-consuming. This study aimed to develop a novel molecular assay for detecting brucellosis. METHODS All complete sequences of chromosome 1 with 2.1-Mbp lengths were compared among all avai...
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ژورنال
عنوان ژورنال: Veterinary Record Open
سال: 2015
ISSN: 2399-2050,2052-6113
DOI: 10.1136/vetreco-2014-000084